Tiger Global Management said Feroz Dewan, head of public equity investing, and another partner are leaving.
Tiger Global runs hedge fund, private equity and long-only stock strategies and has $20 billion in assets under management.
Mr. Dewan, who started at the firm in 2003, plans to start his own business, according to a letter sent to clients Monday. Scott Shleifer, who co-ran Tiger Global's private equity business, will replace him.
Caleb Watts, a partner who joined Tiger Global in 2005, is also departing and plans to manage his own capital.
Tiger Global's private equity business will be run by Lee Fixel, who co-ran the unit with Mr. Shleifer since 2011.
The departures follow those of two other partners, Andrew Bellas and Alexander Captain, who left Tiger Global in January.
Tiger Global also told investors that it will merge Tiger Global Internet Opportunities, a fund started in January with about $700 million, into a larger fund that bets on rising stocks, called Tiger Global Long Opportunities.
Mr. Coleman said in the letter the move would simplify the business. He also said the firm's concentration in Internet, financial technology and software companies will increase in both Tiger Global Investments and the long opportunities fund.
Tiger is offering investors the opportunity to withdraw their money from Tiger Global's public equity funds effective June 15. The firm plans to reopen the funds to a limited amount of capital to replace any redemptions.
Coleman also said in the letter that his firm is looking forward to finding ways to work with Mr. Dewan in the future.